Dr. Steve Valentine, West Virginia University
Host: Young-Jin Lee
Metabolomics analyses performed with liquid chromatography (LC) combined with mass spectrometry (MS) often suffer from an inability to assign dataset features due to the identical mass values of many different isomeric species. Although the recent application of ion mobility spectrometry improves the assignment capabilities, the relatively limited peak capacity of the approach (~100) is still overwhelmed by the sheer number of separate chemical species. Recognizing such assignment limitations, the National Institutes of Health recently issued a FOA for metabolomics centers to develop new assignment technology truly representing a call to arms for the field. Recently my research group has focused our efforts on the development of novel, in-line techniques that features gas- and solution-phase hydrogen/deuterium exchange (HDX) as a means to dramatically enhance compound identification capabilities. These approaches are robust, highly reproducible, and can be predictive. The latter figure of merit is especially encouraging considering the continual introduction of new drugs and their metabolites. The figures of merit of the separate methods as well as their limitations will be discussed.
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